Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Menin Antibodies »

Product Summary for Menin Antibody

Immunogen

A synthetic peptide, which represented a portion of human Multiple Endocrine Neoplasia 1 encoded within exon 11 (MEN1 LocusLink ID 4221).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Menin Antibody

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.
Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399]

Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Menin Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:500-1:5000
Application Notes
This antibody may be used for Western Blot, where a doublet band should be seen at ~72 kDa. Suggested working dilutions: * Western Blot - 1:500-1:5,000 Immunoprecipitation - 2 to 10 ug/mg lysate Immunofluorescence - ND *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Menin

Multiple Endocrine Neoplasia is an autosomal dominant disorder characterized by a high frequency of peptic ulcer disease and primary endocrine abnormalities involving the pituitary, parathyroid, and pancreas.

Long Name

Multiple Endocrine Neoplasia I

Alternate Names

MEAI, MEN1

Entrez Gene IDs

4221 (Human)

Gene Symbol

MEN1

Additional Menin Products

Product Documents for Menin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Menin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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