Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-690

Novus Biologicals, part of Bio-Techne
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NB300-690
NB300-690-0.025mg
NB300-690-0.05mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mer Antibodies »

Product Specifications

Immunogen

Synthetic peptide: corresponding to amino acids 746-757 (CKKIYSGDYYRQG-amide) [p Y749, p Y753, p Y754] of Human MERTK.

Modification

p Tyr754, p Tyr749, p Tyr753

Specificity

MERTK [Tyr749/Tyr753/Tyr754]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Western Blot: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Western Blot: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Western Blot: Mer [p Tyr749, p Tyr753] Antibody [NB300-690] - Phospho & non-Phospho-MerTK with Phospho-MERTK Antibody (NB300-690). Phosphorylated (1) & un-phosphorylated (2, 3) MERTK was immunoprecipitated with MERTK and blotted with Phospho-MerTK antibody (NB300-690). MW of MERTK is 174 kDa. Lower broad band is of IgGs in the immunoprecipitates.
Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - PCaControl cells or PCaGAS6OE cells were starved for 24 hours and then exposed to 10% FBS of culture condition for 1 hour and phosphorylation of Mer was evaluated by (D) immunofluorescence staining. Bar = 50 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - Left panel: Immunofluorescence staining of p-Mer (red)/GAS6 (green) in PCa cells in bone marrow of a PCa patient. Blue, DAPI nuclearstain. Bar = 50 um. Right panel: p-Mer (red)/GAS6 (green) positive cells (white arrows) in the magnification of the white rectangle from left panel. Bar = 20 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.

Applications for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:250

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:750
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mer

MerTK (c-mer, Nyk, Eyk) is a tyrosine kinase that belongs to a family of transmembrane receptors (1). MerTk has been implicated in reversible cell growth arrest, survival, proliferation and cell adhesion. Growth arrest specific protein 6 (Gas6) is an activating ligand for MerTK (2-3). MerTK is required for clearance of apoptotic cells by mononuclear phagocytes in mice; with its absence resulting in progressive lupus like autoimmunity (4). Defects in MERTK are a cause of retinitis pigmentosa (RP) (5).

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human)

Gene Symbol

MERTK

UniProt

Q12866

Additional Mer Products

Product Documents for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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