Mer [p Tyr754, p Tyr749, p Tyr753] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-690
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide: corresponding to amino acids 746-757 (CKKIYSGDYYRQG-amide) [p Y749, p Y753, p Y754] of Human MERTK.
Modification
p Tyr754, p Tyr749, p Tyr753
Specificity
MERTK [Tyr749/Tyr753/Tyr754]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody
Western Blot: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]
Western Blot: Mer [p Tyr749, p Tyr753] Antibody [NB300-690] - Phospho & non-Phospho-MerTK with Phospho-MERTK Antibody (NB300-690). Phosphorylated (1) & un-phosphorylated (2, 3) MERTK was immunoprecipitated with MERTK and blotted with Phospho-MerTK antibody (NB300-690). MW of MERTK is 174 kDa. Lower broad band is of IgGs in the immunoprecipitates.Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]
Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - PCaControl cells or PCaGAS6OE cells were starved for 24 hours and then exposed to 10% FBS of culture condition for 1 hour and phosphorylation of Mer was evaluated by (D) immunofluorescence staining. Bar = 50 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]
Immunocytochemistry/Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690] - Left panel: Immunofluorescence staining of p-Mer (red)/GAS6 (green) in PCa cells in bone marrow of a PCa patient. Blue, DAPI nuclearstain. Bar = 50 um. Right panel: p-Mer (red)/GAS6 (green) positive cells (white arrows) in the magnification of the white rectangle from left panel. Bar = 20 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.8365), licensed under a CC-BY license.Applications for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:250
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:250
Western Blot
1:750
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Mer
Long Name
Receptor Tyrosine Protein Kinase Mer
Alternate Names
c-Eyk, C-mer, MerTK
Entrez Gene IDs
10461 (Human)
Gene Symbol
MERTK
UniProt
Additional Mer Products
Product Documents for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody
Product Specific Notices for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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