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Methionine Sulfoxide Reductase B Antibody (8B2)

Novus Biologicals, part of Bio-Techne | Catalog # H00051734-M02

Novus Biologicals, part of Bio-Techne
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H00051734-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8B2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SEPX1 (NP_057416, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLN

Specificity

SEPX1 (8B2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Methionine Sulfoxide Reductase B Antibody (8B2)

Western Blot: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

Western Blot: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

Western Blot: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02] - Analysis of SEPX1 expression in transfected 293T cell line by SEPX1 monoclonal antibody (M02), clone 8B2.Lane 1: SEPX1 transfected lysate(12.713 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

Immunohistochemistry-Paraffin: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

Immunohistochemistry-Paraffin: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02] - Analysis of monoclonal antibody to SEPX1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1.5 ug/ml.
ELISA: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

ELISA: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02]

ELISA: Methionine Sulfoxide Reductase B Antibody (8B2) [H00051734-M02] - Detection limit for recombinant GST tagged SEPX1 is approximately 0.1ng/ml as a capture antibody.

Applications for Methionine Sulfoxide Reductase B Antibody (8B2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Methionine Sulfoxide Reductase B

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase B (MsrB) family, and it is expressed in a variety of adult and fetal tissues.

Alternate Names

EC 1.8.4.-, methionine sulfoxide reductase, methionine-R-sulfoxide reductase B1, MGC3344, MsrB1, MSRB1selenoprotein R, Selenoprotein X, selenoprotein X, 1, SELR, SelX

Entrez Gene IDs

51734 (Human)

Gene Symbol

MSRB1

OMIM

606216 (Human)

Additional Methionine Sulfoxide Reductase B Products

Product Documents for Methionine Sulfoxide Reductase B Antibody (8B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methionine Sulfoxide Reductase B Antibody (8B2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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