Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Methionyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 175 to 225 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionyl tRNA synthetase Antibody - BSA Free

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312]

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312]

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312] - FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (NBP2-22312) left image and (NBP2-22313) right image, used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22312 used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody NBP2-22313. For blotting immunoprecipitated MARS, NBP2-22312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for Methionyl tRNA synthetase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Methionyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Alternate Names

cytosolic methionyl-tRNA synthetase, EC 6.1.1.10, FLJ35667, methionine tRNA ligase 1, cytoplasmic, Methionine--tRNA ligase, methionyl-tRNA synthetase, methionyl-tRNA synthetase, cytoplasmic, MetRS, MRS, MTRNS

Gene Symbol

MARS1

UniProt

NP_004981.2 (Human)

Additional Methionyl tRNA synthetase Products

Product Documents for Methionyl tRNA synthetase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methionyl tRNA synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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