METTL1 Antibody (9C2F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06651
![Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]](https://resources.bio-techne.com/images/products/METTL1-Antibody-9C2F2-Western-Blot-NBP3-06651-img0001.jpg "Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA Detection, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9C2F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (Accession#: NP_005362.3; Asp 32-Gln 265).
Reactivity Notes
No cross-reactivity in ELISA with:
E.coli cell lysate
E.coli cell lysate
Specificity
No cross-reactivity in ELISA with: E.coli cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for METTL1 Antibody (9C2F2)
Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]
Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - NBP3-06651 at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 31 kDa.![Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]](https://resources.bio-techne.com/images/products/METTL1-Antibody-9C2F2-Western-Blot-NBP3-06651-img0002.jpg "Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]")
Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]
Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 2 uL NBP3-06651 and 15 ul of 50 % Protein G agarose.Primary antibody: NBP3-06651 at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 34 kDa.Applications for METTL1 Antibody (9C2F2)
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Application Notes
This antibody will detect Human METTL1 in ELISA pair set (Catalog: # NBP2-79646). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP3-06375).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: METTL1
Alternate Names
C12orf1, D1075-like gene product, EC 2.1.1.33, FLJ95748, methyltransferase like 1, methyltransferase-like 1, Methyltransferase-like protein 1, TRM8, tRNA (guanine-N(7)-)-methyltransferase, tRNA(m7G46)-methyltransferase, YDL201w
Gene Symbol
METTL1
Additional METTL1 Products
Product Documents for METTL1 Antibody (9C2F2)
Product Specific Notices for METTL1 Antibody (9C2F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...