METTL1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99811
![Western Blot: METTL1 Antibody [NBP2-99811]](https://resources.bio-techne.com/images/products/METTL1-Antibody-Western-Blot-NBP2-99811-img0002.jpg "Western Blot: METTL1 Antibody [NBP2-99811]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human METTL1 (Accession#: NP_005362.3; Asp 32-Gln 265)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for METTL1 Antibody
Western Blot: METTL1 Antibody [NBP2-99811]
Western Blot: METTL1 Antibody [NBP2-99811] - Anti-METTL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).![Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811]](https://resources.bio-techne.com/images/products/METTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-99811-img0003.jpg "Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811]")
Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811]
Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811] - Immunochemical staining of human METTL1 in human bladder carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated wit![Immunoprecipitation: METTL1 Antibody [NBP2-99811]](https://resources.bio-techne.com/images/products/METTL1-Antibody-Immunoprecipitation-NBP2-99811-img0004.jpg "Immunoprecipitation: METTL1 Antibody [NBP2-99811]")
Immunoprecipitation: METTL1 Antibody [NBP2-99811]
Immunoprecipitation: METTL1 Antibody [NBP2-99811] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate0.5 ul anti-METTL1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-METTL1 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDaApplications for METTL1 Antibody
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunohistochemistry-Paraffin
1:2000-1:10000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: METTL1
Alternate Names
C12orf1, D1075-like gene product, EC 2.1.1.33, FLJ95748, methyltransferase like 1, methyltransferase-like 1, Methyltransferase-like protein 1, TRM8, tRNA (guanine-N(7)-)-methyltransferase, tRNA(m7G46)-methyltransferase, YDL201w
Gene Symbol
METTL1
Additional METTL1 Products
Product Documents for METTL1 Antibody
Product Specific Notices for METTL1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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