METTL4 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04490

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all METTL4 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3). MSVVHQLSAGWLLDHLSFINKINYQLHQHHEPCCRKKEFTTSVHFESLQMDSVSSSGVCAAFIASDSSTKPENDDGGNYEMFTRKFVFRPELFDVTKPYITPAVHKECQQSNEKEDLMNGVKKEISISIIGKKRKRCVVFNQGELDAMEYHTKIRELILDGSLQLIQEGLKSGFLYPLFEKQDKGSKPIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for METTL4 Antibody - Azide and BSA Free

Western Blot: METTL4 AntibodyAzide and BSA Free [NBP3-04490]

Western Blot: METTL4 AntibodyAzide and BSA Free [NBP3-04490]

Western Blot: METTL4 Antibody [NBP3-04490] - Analysis of extracts of various cell lines, using METTL4 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunohistochemistry-Paraffin: METTL4 Antibody - Azide and BSA Free [NBP3-04490]

Immunohistochemistry-Paraffin: METTL4 Antibody - Azide and BSA Free [NBP3-04490]

Immunohistochemistry-Paraffin: METTL4 Antibody [NBP3-04490] - Rat testis using METTL4 Rabbit pAb (NBP3-04490) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: METTL4 Antibody - Azide and BSA Free [NBP3-04490]

Immunocytochemistry/ Immunofluorescence: METTL4 Antibody - Azide and BSA Free [NBP3-04490]

Immunocytochemistry/Immunofluorescence: METTL4 Antibody [NBP3-04490] - Immunofluorescence analysis of NIH/3T3 cells using METTL4 Rabbit pAb (NBP3-04490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for METTL4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: METTL4

Probable methyltransferase

Alternate Names

EC 2.1.1.-, FLJ23017, HsT661, methyltransferase like 4, methyltransferase-like protein 4, MGC117235

Gene Symbol

METTL4

Additional METTL4 Products

Product Documents for METTL4 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for METTL4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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