MFF Antibody (HL1311) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25563
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1311
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MFF Antibody (HL1311) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] -
Immunocytochemistry/Immunofluorescence: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:4500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] -
Immunohistochemistry-Paraffin: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. MFF stained by MFF antibody [HL1311] (NBP3-25563) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] -
Western Blot: MFF Antibody (HL1311) - Azide and BSA Free [NBP3-25563] - U87-MG whole cell and membrane extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1311] (NBP3-25563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for MFF Antibody (HL1311) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MFF
Alternate Names
mitochondrial fission factor
Gene Symbol
MFF
Additional MFF Products
Product Documents for MFF Antibody (HL1311) - Azide and BSA Free
Product Specific Notices for MFF Antibody (HL1311) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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