MFG-E8 Antibody (EDM45)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44412

Novus Biologicals, part of Bio-Techne
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NBP2-44412-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # EDM45

Concentration

0.2 mg/ml

View all MFG-E8 Antibodies »

Product Specifications

Immunogen

Delipidated human milk fat globule membrane preparation

Localization

Cell Surface & Cytoplasmic

Marker

Breast Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47813)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MFG-E8 Antibody (EDM45)

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412] - Formalin-fixed, paraffin-embedded human Pancreas stained with MFG-E8 Antibody (EDM45)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412] - Human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (EDM45)
Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412]

Immunohistochemistry-Paraffin: MFG-E8 Antibody (EDM45) [NBP2-44412] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with MFG-E8 Antibody (EDM45)
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Applications for MFG-E8 Antibody (EDM45)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MFG-E8

Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.

Long Name

Milk Fat Globule EGF Factor 8

Alternate Names

BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1

Gene Symbol

MFGE8

UniProt

Q08431

Additional MFG-E8 Products

Product Documents for MFG-E8 Antibody (EDM45)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MFG-E8 Antibody (EDM45)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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