MGAT4A Antibody (4H4)
Novus Biologicals, part of Bio-Techne | Catalog # H00011320-M02
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4H4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MGAT4A (NP_036346, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVNVESYLFHSGNQEHPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIGKFENGVAEGMVDPSLNPISAFRLSVIQNSAVWAILNEIHIKKATN
Reactivity Notes
Other species not tested. Rat reactivity reported in scientific literature (PMID: 26104473)
Specificity
MGAT4A - mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MGAT4A Antibody (4H4)
![Western Blot: MGAT4A Antibody (4H4) [H00011320-M02]](https://resources.bio-techne.com/images/products/MGAT4A-Antibody-4H4-Western-Blot-H00011320-M02-img0008.jpg "Western Blot: MGAT4A Antibody (4H4) [H00011320-M02]")
Western Blot: MGAT4A Antibody (4H4) [H00011320-M02]
Western Blot: MGAT4A Antibody (4H4) [H00011320-M02] - MGAT4A monoclonal antibody (M02), clone 4H4 Analysis of MGAT4A expression in Jurkat.![Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02]](https://resources.bio-techne.com/images/products/MGAT4A-Antibody-4H4-Immunohistochemistry-Paraffin-H00011320-M02-img0006.jpg "Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02]")
Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02]
Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02] - Analysis of monoclonal antibody to MGAT4A on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.2 ug/ml.
Sandwich ELISA: MGAT4A Antibody (4H4) [H00011320-M02] - Detection limit for recombinant GST tagged MGAT4A is approximately 0.1ng/ml as a capture antibody.
Applications for MGAT4A Antibody (4H4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MGAT4A
Long Name
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
Alternate Names
EC 2.4.1.145, GlcNAc-T IVa, GnT-4a, GNT-IV, GNT-IVA, N-Acetylglucosaminyltransferase IVa
Entrez Gene IDs
11320 (Human)
Gene Symbol
MGAT4A
OMIM
604623 (Human)
UniProt
Additional MGAT4A Products
Product Documents for MGAT4A Antibody (4H4)
Product Specific Notices for MGAT4A Antibody (4H4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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