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MGAT4A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17285

Novus Biologicals, part of Bio-Techne
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NBP2-17285

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MGAT4A. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque.

Localization

Alpha-1; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MGAT4A Antibody

Western Blot: MGAT4A Antibody [NBP2-17285]

Western Blot: MGAT4A Antibody [NBP2-17285]

Western Blot: MGAT4A Antibody [NBP2-17285] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MGAT4A Antibody [NBP2-17285]

Immunocytochemistry/ Immunofluorescence: MGAT4A Antibody [NBP2-17285]

Immunocytochemistry/Immunofluorescence: MGAT4A Antibody [NBP2-17285] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MGAT4A Antibody [NBP2-17285]

Immunohistochemistry-Paraffin: MGAT4A Antibody [NBP2-17285]

Immunohistochemistry-Paraffin: MGAT4A Antibody [NBP2-17285] - Immunohistochemical analysis of paraffin-embedded H358 xenograft, using antibody at 1:250 dilution.

Applications for MGAT4A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MGAT4A

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq]

Long Name

alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Alternate Names

EC 2.4.1.145, GlcNAc-T IVa, GnT-4a, GNT-IV, GNT-IVA, N-Acetylglucosaminyltransferase IVa

Gene Symbol

MGAT4A

UniProt

Additional MGAT4A Products

Product Documents for MGAT4A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MGAT4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.