MgcRacGAP/RACGAP1 Antibody (1G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00029127-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Chicken

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MgcRacGAP Antibodies »

Product Specifications

Immunogen

RACGAP1 (AAH32754, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCD

Reactivity Notes

Other species not tested.

Localization

Cytoplasmic and Nuclear

Specificity

RACGAP1 - Rac GTPase activating protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for MgcRacGAP/RACGAP1 Antibody (1G6)

Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48?h, cells were immunostained for midbody markers (green), Beta-tubulin (red), and DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-0561-6) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01]

Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Analysis of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
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Applications for MgcRacGAP/RACGAP1 Antibody (1G6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MgcRacGAP

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]

Long Name

Male Germ Cell Rac GTPase-activating Protein

Alternate Names

RACGAP1

Entrez Gene IDs

29127 (Human)

Gene Symbol

RACGAP1

OMIM

604980 (Human)

UniProt

AAH32754 (Human)

Additional MgcRacGAP Products

Product Documents for MgcRacGAP/RACGAP1 Antibody (1G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MgcRacGAP/RACGAP1 Antibody (1G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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