MgcRacGAP/RACGAP1 Antibody (JE41-19)

Western Blot: MgcRacGAP/RACGAP1 Antibody (JE41-19) [NBP3-32889]

Western Blot: MgcRacGAP/RACGAP1 Antibody (JE41-19) [NBP3-32889] - Western blot analysis of MgcRacGAP/RACGAP1 on different lysates with Rabbit anti-MgcRacGAP/RACGAP1 antibody (NBP3-32889) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lane 5: PC-12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 71 kDa
Observed band size: 71 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32889) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.