Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S75-3

Concentration

1 mg/ml

View all mGluR5/1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Localization

Cell Membrane

Specificity

Detects approx 130kDa (both mGluR1 and mGluR5).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for mGluR5/1 Antibody (S75-3)

Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5/1 protein using Mouse Anti-mGluR5/1 Monoclonal Antibody, Clone S75-33 (NBP2-22424). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5/1 Monoclonal Antibody (NBP2-22424) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.
Immunocytochemistry/ Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Immunocytochemistry/ Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-22424) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Immunocytochemistry/ Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424]

Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (NBP2-22424) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) mGluR1/5 glutamate receptor Antibody. (D) Composite.

Applications for mGluR5/1 Antibody (S75-3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of mGluR1/5 glutamate receptor Antibody was sufficient for detection of mGluR1/5 glutamate receptor in 20 ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mGluR5/1

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Alternate Names

glutamate receptor, metabotropic 5, GPRC1EMGLUR5metabotropic glutamate receptor 5, mGlu5, mGluR5

Gene Symbol

GRM5

UniProt

P31424 (Rat)

Additional mGluR5/1 Products

Product Documents for mGluR5/1 Antibody (S75-3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for mGluR5/1 Antibody (S75-3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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