Skip to main content

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S75-33

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for mGluR5/1a Antibody (S75-33)

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Localization

Cell Membrane

Specificity

Detects 130kDa (both mGluR1 and mGluR5).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for mGluR5/1a Antibody (S75-33)

Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5/1a Glutamate Receptor protein using Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody, Clone S75-33 (NBP2-59334). Primary Antibody: Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody (NBP2-59334) at 1:1000.
Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33 (NBP2-59334). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-59334) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Immunocytochemistry/ Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334]

Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.

Applications for mGluR5/1a Antibody (S75-33)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: mGluR5/1a

Long Name

Metabotropic Glutamate Receptor 5 and 1

Additional mGluR5/1a Products

Product Documents for mGluR5/1a Antibody (S75-33)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mGluR5/1a Antibody (S75-33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...