mGluR6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-189

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all mGluR6 Antibodies »

Product Specifications

Immunogen

C-terminus of rat metabotropic Glutamate Receptor 6 (mGluR6)

Reactivity Notes

Reactivity has been poor on human brain tissue.

Localization

Integral membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mGluR6 Antibody

Western Blot: mGluR6 Antibody [NB300-189]

Western Blot: mGluR6 Antibody [NB300-189]

Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: mGluR6 Antibody [NB300-189]

Immunocytochemistry/ Immunofluorescence: mGluR6 Antibody [NB300-189]

Immunocytochemistry/Immunofluorescence: mGluR6 Antibody [NB300-189] - metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red). DAPI was used to counterstain cell nuclei.

Applications for mGluR6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:1000
Application Notes
In Western blot, a band is seen at ~96 kDa. Neurons of expected brain regions stain well

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mGluR6

Metabotropic Glutamate Receptor 6 (Mglur6 or Grm6), member of class III of Mglur family/GPCR3 family, is a multi-pass membrane protein localized in cell membrane with restricted expression in retinal inner nuclear layer on dendrites of ON-bipolar cells wherein it interact with effector G protein G0 alpha and act as a receptor for glutamate. An increment of light intensity in retina hyperpolarizes the photoreceptor to set off two opposing signals in OFF and ON bipolar cells, whereas in darkness, glutamate released by photoreceptors depolarizes OFF bipolar cells with hyperpolarization of ON bipolar cells. This ON bipolar cell "sign inverting" cascade involves glutamate mediated activation of Mglur6 leading to G0 alpha stimulation which results in closure of a presumably constitutively active nonselective cation channel TRPM1 (transient receptor potential melastatin 1). mGluR6 activity is mediated by a G-protein that inhibits adenylate cyclase activity and mGluR6 is the only known mGluR that mediates direct synaptic transmission in the nervous system with its role in mediating ON-response in vertebrate retinal ON-pathway. Genetic inactivation of mGluR6 in murine model blocks the ON-response and in humans, mutations in the mGluR6 gene have been linked to congenital stationary night blindness, characterized in the electroretinogram by the absence of an ON response.

Long Name

Metabotropic Glutamate Receptor 6

Alternate Names

Gm3, GPRC1F, GRM6

Gene Symbol

GRM6

Additional mGluR6 Products

Product Documents for mGluR6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for mGluR6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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