MGMT Antibody (10G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26141
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MGMT Antibody (10G7)
Immunogen
A synthesized peptide derived from Human MGMT [UniProt P16455]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MGMT Antibody (10G7)
Immunohistochemistry: MGMT Antibody (10G7) [NBP3-26141] -
Immunohistochemistry: MGMT Antibody (10G7) [NBP3-26141] - Image of MGMT Antibody (10G7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: MGMT Antibody (10G7) [NBP3-26141] -
Western Blot: MGMT Antibody (10G7) [NBP3-26141] - Positive Western Blot detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate.All lanes: MGMT Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Applications for MGMT Antibody (10G7)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: MGMT
MGMT antibodes are useful tools for DNA repair studies and for some cancer research.
Long Name
O-6 Methylguanine-DNA Methyltransferase
Alternate Names
6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Gene Symbol
MGMT
Additional MGMT Products
Product Documents for MGMT Antibody (10G7)
Product Specific Notices for MGMT Antibody (10G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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