MGMT Antibody (MT 23.2) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-168B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-168AF350

Alexa Fluor 405

NB100-168AF405

Alexa Fluor 488

NB100-168AF488

Alexa Fluor 532

NB100-168AF532

Alexa Fluor 594

NB100-168AF594

Alexa Fluor 647

NB100-168AF647

Alexa Fluor 700

NB100-168AF700

Alexa Fluor 750

NB100-168AF750

Allophycocyanin

NB100-168APC

CoraFluor 1

NB100-168CL1

DyLight 350

NB100-168UV

DyLight 405

NB100-168V

DyLight 488

NB100-168G

DyLight 550

NB100-168R

DyLight 594

NB100-168DL594

DyLight 650

NB100-168C

DyLight 680

NB100-168FR

DyLight 755

NB100-168IR

FITC

NB100-168F

HRP

NB100-168H

Janelia Fluor 525

NB100-168JF525

Janelia Fluor 549

NB100-168JF549

Janelia Fluor 585

NB100-168JF585

Janelia Fluor 635

NB100-168JF635

Janelia Fluor 646

NB100-168JF646

Janelia Fluor 669

NB100-168JF669

mFluor Violet 450 SE

NB100-168MFV450

mFluor Violet 500 SE

NB100-168MFV500

mFluor Violet 610 SE

NB100-168MFV610

PE

NB100-168PE

PerCP

NB100-168PCP

Forumulation

Catalog #

BSA Free

NB100-168

Azide and BSA Free

NBP2-80847

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG_2B Clone # MT 23.2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MGMT Antibodies »

Product Summary for MGMT Antibody (MT 23.2) [Biotin]

Immunogen

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]

Reactivity Notes

This antibody is mouse reactivity negative.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for MGMT Antibody (MT 23.2) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MGMT

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Alternate Names

6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene Symbol

MGMT

Additional MGMT Products

Product Documents for MGMT Antibody (MT 23.2) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MGMT Antibody (MT 23.2) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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