Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Bovine, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2G5

Concentration

1.0 mg/ml

View all MHC Class I Antibodies »

Product Specifications

Immunogen

Purified H-2Kb and H-2Db MHC-I molecules

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Chinese Hamster reactivity reported in scientific literature (PMID: 30441765).

Specificity

NB100-65938 recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for MHC Class I Antibody (2G5)

Application
Recommended Usage

Flow Cytometry

1:10-1:25

Immunoprecipitation

1:10-1:500
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 10 ul.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class I

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (2G5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MHC Class I Antibody (2G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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