Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A4

Format

BSA Free

Concentration

0.5 mg/ml

View all MHC Class I Antibodies »

Product Summary for MHC Class I Antibody (A4) - BSA Free

Immunogen

The immunogen for this antibody was HLA MHC Class I free chain without B2 microglobulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for MHC Class I Antibody (A4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated PBMC. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MHC Class I

The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (beta2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (A4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MHC Class I Antibody (A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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