MHC Class I Antibody (ER-HR52) [Janelia Fluor® 549]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64952JF549
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Janelia Fluor 549
Antibody Source
Monoclonal Rat IgG2A Clone # ER-HR52
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Adherent FI (CBAXBL) bone marrow stromal cells
Reactivity Notes
0
Localization
Cell Membrane; Type I membrane protein.
Specificity
NB100-64952 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP52 specifically recognizes mouse strains with the haplotypes H-2b,w16 and H-2d,p,q. Mouse strains with the haplotypes H-2f,r,s,w17,w23,w27 show weak reactivity with this antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for MHC Class I Antibody (ER-HR52) [Janelia Fluor® 549]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MHC Class I
Alternate Names
DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1
Gene Symbol
HLA-E
Additional MHC Class I Products
Product Documents for MHC Class I Antibody (ER-HR52) [Janelia Fluor® 549]
Product Specific Notices for MHC Class I Antibody (ER-HR52) [Janelia Fluor® 549]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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