MHC Class I Antibody (MEM-E/06) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-307
![Immunohistochemistry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]](https://resources.bio-techne.com/images/products/MHC-Class-I-Antibody-MEM-E-06-Immunohistochemistry-NB500-307-img0001.jpg "Immunohistochemistry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-E/06
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for MHC Class I Antibody (MEM-E/06) - BSA Free
Immunogen
Bacterially expressed recombinant full length protein refolded with beta2-microglobulin and peptide (Human).
Localization
Type I membrane protein.
Specificity
This antibody (clone MEM-E/06) recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. Some resultss showed that this antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, others have confirmed that this antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MHC Class I Antibody (MEM-E/06) - BSA Free
Immunohistochemistry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]
Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).![Flow Cytometry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]](https://resources.bio-techne.com/images/products/MHC-Class-I-Antibody-MEM-E-06-Flow-Cytometry-NB500-307-img0002.jpg "Flow Cytometry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]")
Flow Cytometry: MHC Class I Antibody (MEM-E/06) - BSA Free [NB500-307]
Flow Cytometry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Analysis using PE conjugate of NB500-307. A cell surface stain was performed on THP-1 cells with MHC Class I antibody (MEM-E/06) NB500-307 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.Applications for MHC Class I Antibody (MEM-E/06) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:50
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC Class I
Alternate Names
DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1
Entrez Gene IDs
3133 (Human)
Gene Symbol
HLA-E
Additional MHC Class I Products
Product Documents for MHC Class I Antibody (MEM-E/06) - BSA Free
Product Specific Notices for MHC Class I Antibody (MEM-E/06) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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