Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # ER-TR2

Concentration

1.0 mg/ml

View all MHC Class II Antibodies »

Product Specifications

Immunogen

Isolated C3H thymic stromal cells

Localization

Type I membrane protein. Localizes to late endocytic compartment. Associates with lysosome membranes.

Specificity

NB100-64959 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class II molecule. In the mouse, MHC class II molecules are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR2 stains cortical and medullary stromal cells. Clone ER-TR2 specifically recognizes mouse strains with the haplotypes I-A k,s,r. Mouse strains with the haplotypes I-A b,d,q,f are not recognized by this antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for MHC Class II Antibody (ER-TR2)

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice reared in a pathogen free environment for the purposed of comparing with mice reared in standard living conditions.
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Comparison of MHC II levels in retinal microglia from mice without transplanted GFP+ BM Cells (panel A) and from mice with transplanted GFP+ BM cells.
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) - BSA Free [NB100-64959]

Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice kept in standard living conditions for the purpose of comparing to MHC II expression over time in mice kept in pathogen free environment.

Applications for MHC Class II Antibody (ER-TR2)

Application
Recommended Usage

Flow Cytometry

1:100-1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 23750207).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class II

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Entrez Gene IDs

14998 (Mouse)

Gene Symbol

HLA-DMA

Additional MHC Class II Products

Product Documents for MHC Class II Antibody (ER-TR2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MHC Class II Antibody (ER-TR2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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