MHC Class II Antibody (M5/114) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21789
![Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-Azide-Free-Flow-Cytometry-NBP2-21789-img0001.jpg "Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # M5/114
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Activated C57BL/6 mouse spleen cells
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34166814).
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for MHC Class II Antibody (M5/114) - Azide and BSA Free
Flow Cytometry: MHC Class II Antibody (M5/114) - Azide and BSA Free [NBP2-21789]
Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.Applications for MHC Class II Antibody (M5/114) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Frozen
1:50-1:300
Immunohistochemistry-Paraffin
1:50-1:300
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC Class II
Alternate Names
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbol
HLA-DMA
Additional MHC Class II Products
Product Documents for MHC Class II Antibody (M5/114) - Azide and BSA Free
Product Specific Notices for MHC Class II Antibody (M5/114) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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