MHC class II (I-A/I-E) Antibody (MK-D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07531

Novus Biologicals, part of Bio-Techne
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NBP3-07531-100ug
NBP3-07531-20ug
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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # MK-D6

Concentration

0.2 mg/ml

View all MHC class II (I-A/I-E) Antibodies »

Product Specifications

Immunogen

Spleen cells from B10.D2 mice

Reactivity Notes

Does not react with Human.

Localization

Cell Surface and Cytoplasmic

Specificity

The antibody is directed against Mouse major histocompatibility complex class II, I-A d. It does not react with I-Ak, I-Ab, I-As, I-Af or I-Aa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08856)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for MHC class II (I-A/I-E) Antibody (MK-D6)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MHC class II (I-A/I-E)

Major Histocompatibility Complex (MHC) class II is a heterodimeric transmembrane protein that is expressed on antigen-presenting cells including dendritic cells, macrophages, and B cells. Mouse MHC class II is expressed as multiple isotypes encoded by four genes located in the mouse MHC. MHC class II alpha and beta subunits are assembled into heterodimers in the endoplasmic reticulum, where they are complexed to an invariant chain (Ii). Following synthesis, MHC class II is further processed in the MHC class II compartment, a specialized endosome. Here, the Ii chain is digested and peptide antigens derived from extracellular proteins are loaded into the groove between the alpha and beta MHC class II subunits. Antigens presented on MHC class II bind to epitope-specific T Cell Receptors (TCR) expressed by CD4+ helper T cells. In addition to presenting peptide antigens to T cells, MHC class II can also initiate intracellular signaling pathways in antigen-presenting cells that modulate their proliferation, maturation, and apoptosis.

Long Name

Major Histocompatibility Complex Class II IA and IE

Alternate Names

H2-Ea, MHC class II IA/IE

Gene Symbol

H2-Ea

UniProt

P01904 (Mouse), P04224 (Mouse)

Additional MHC class II (I-A/I-E) Products

Product Documents for MHC class II (I-A/I-E) Antibody (MK-D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MHC class II (I-A/I-E) Antibody (MK-D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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