MICALL1 Antibody

Western Blot: MICALL1 Antibody [H00085377-B01P]

Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in HepG2.

Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody [H00085377-B01P] -

Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. (A–C) HeLa cells were incubated with methanol (vehicle) or BFA (5 µg/ml) for 5 or 15 min. (A) Percentage of cells with endogenous (endo.) WDR44 tubules. n = 7–25. (B) Confocal images of cells stained with alpha-WDR44 & alpha-TGN46 or preincubated with Alexa Fluor 546–Transferrin (10 µg/ml, 1 h). (C) Manders colocalization coefficients for the indicated proteins with endogenous WDR44 structures. n = 10–50 cells. (D) Confocal images of transfected HeLa cells showing colocalization of RFP-WDR44 with GFP-STX16 & GFP-VAMP3. (E) Immunoprecipitation (IP) of transfected 293T cells with alpha-GFP. uGRAF2 was coimmunoprecipitated by GFP-MICAL1, but not by any other member of the MICAL family. (F) Confocal images of transfected HeLa cells stained with alpha-WDR44. In the case of MICAL3pF1KA0819 & MICAL-L2, boxed areas show tubules positive only for WDR44 (red), only for MICAL3pF1KA0819/MICAL-L2 (cyan), or shared by the two proteins (white). (G) Percentage of transfected HeLa cells with endogenous (endo.) WDR44 tubules. n = 4. (H) Confocal images of untransfected HeLa cells stained with alpha-WDR44 & alpha-MICAL-L1. Boxed areas show tubules positive only for WDR44 (red), only for MICAL-L1 (cyan), or shared by the two proteins (white). (I) Internalized Integrin-beta 1 in shRNA-expressing HeLa cells after uptake of alpha-Integrin-beta 1 & following a 4-h chase. n = 4. (A, C, G, & I) Data are means ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; & ****, P < 0.0001. (B, D, F, & H) Insets show magnifications of the boxed areas. Scale bars: 10 µm; scale bars of insets: 2 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32344433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.