MICALL1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70017
![Western Blot: MICALL1 AntibodyBSA Free [NBP2-70017]](https://resources.bio-techne.com/images/products/MICALL1-Antibody-Western-Blot-NBP2-70017-img0003.jpg "Western Blot: MICALL1 AntibodyBSA Free [NBP2-70017]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry (Negative), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for MICALL1 Antibody - BSA Free
Immunogen
Synthetic peptide made to an internal portion of the human MICALL1 protein (between amino acids 200-280) [UniProt Q8N3F8]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MICALL1 Antibody - BSA Free
Western Blot: MICALL1 AntibodyBSA Free [NBP2-70017]
Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody - BSA Free [NBP2-70017]](https://resources.bio-techne.com/images/products/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-70017-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody - BSA Free [NBP2-70017]")
Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody - BSA Free [NBP2-70017]
Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-70017] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MICALL1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: MICALL1 Antibody - BSA Free [NBP2-70017]](https://resources.bio-techne.com/images/products/MICALL1-Antibody-Immunohistochemistry-Paraffin-NBP2-70017-img0002.jpg "Immunohistochemistry-Paraffin: MICALL1 Antibody - BSA Free [NBP2-70017]")
Immunohistochemistry-Paraffin: MICALL1 Antibody - BSA Free [NBP2-70017]
Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP2-70017] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human testis using 1:200 dilution of MICALL1 antibody (NBP2-70017). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a moderate whole cell with nuclear staining.Applications for MICALL1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: MICALL1
Alternate Names
DKFZp686M2226, KIAA1668, MICAL-L1, MICAL-like 1, MICAL-like protein 1
Gene Symbol
MICALL1
Additional MICALL1 Products
Product Documents for MICALL1 Antibody - BSA Free
Product Specific Notices for MICALL1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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