MID1 Antibody (2C11)

Novus Biologicals, part of Bio-Techne | Catalog # H00004281-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2C11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MID1 Antibodies »

Product Specifications

Immunogen

MID1 (AAH53626, 441 a.a. ~ 540 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGR

Specificity

midline 1 (Opitz/BBB syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MID1 Antibody (2C11)

Western Blot: MID1 Antibody (2C11) [H00004281-M06]

Western Blot: MID1 Antibody (2C11) [H00004281-M06]

Western Blot: MID1 Antibody (2C12) [H00004281-M06] - detection against Immunogen (37 KDa) .

Applications for MID1 Antibody (2C11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MID1

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined.

Alternate Names

EC 6.3.2.-, FLJ57031, FLJ58683, FLJ76288, FXYGBBB1, Midin, midline 1 (Opitz/BBB syndrome), RNF59MIDIN, TRIM18mouse, homolog of

Entrez Gene IDs

4281 (Human)

Gene Symbol

MID1

OMIM

300000 (Human)

UniProt

AAH53626 (Human)

Additional MID1 Products

Product Documents for MID1 Antibody (2C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MID1 Antibody (2C11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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