MID2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00011043-B02P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MID2 Antibodies »

Product Specifications

Immunogen

MID2 (NP_438112.1, 1 a.a. - 685 a.a.) full-length human protein. METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Specificity

MID2 - midline 2,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for MID2 Antibody

Western Blot: MID2 Antibody [H00011043-B02P]

Western Blot: MID2 Antibody [H00011043-B02P]

Western Blot: MID2 Antibody [H00011043-B02P] - Analysis of MID2 expression in transfected 293T cell line by MID2 polyclonal antibody. Lane 1: MID2 transfected lysate(75.35 KDa). Lane 2: Non-transfected lysate.

Applications for MID2 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MID2

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

EC 6.3.2.-, FLJ37715, FLJ41813, FXY2midin-2, midin 2, Midin-2, midline 2, Midline defect 2, Midline-2, RING finger protein 60, RNF60midline-2, TRIM1probable E3 ubiquitin-protein ligase MID2, tripartite motif protein 1, Tripartite motif-containing protein 1

Entrez Gene IDs

11043 (Human)

Gene Symbol

MID2

UniProt

NP_438112.1 (Human)

Additional MID2 Products

Product Documents for MID2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MID2 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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