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MIDN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30437

Novus Biologicals, part of Bio-Techne
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NBP2-30437
NBP2-30437-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTTSCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVER

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIDN Antibody

Western Blot: MIDN Antibody [NBP2-30437]

Western Blot: MIDN Antibody [NBP2-30437]

Western Blot: MIDN Antibody [NBP2-30437] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2
Immunocytochemistry/ Immunofluorescence: MIDN Antibody [NBP2-30437]

Immunocytochemistry/ Immunofluorescence: MIDN Antibody [NBP2-30437]

Immunocytochemistry/Immunofluorescence: MIDN Antibody [NBP2-30437] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry: MIDN Antibody [NBP2-30437]

Immunohistochemistry: MIDN Antibody [NBP2-30437]

Immunohistochemistry: MIDN Antibody [NBP2-30437] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Applications for MIDN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIDN

MIDN may be involved in regulation of genes related to neurogenesis in the nucleolus

Alternate Names

DKFZp547M072, Midbrain nucleolar protein, midnolin

Gene Symbol

MIDN

UniProt

Additional MIDN Products

Product Documents for MIDN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MIDN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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