MIDN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30437
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTTSCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVER
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MIDN Antibody
Western Blot: MIDN Antibody [NBP2-30437]
Western Blot: MIDN Antibody [NBP2-30437] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line NTERA-2
Immunocytochemistry/ Immunofluorescence: MIDN Antibody [NBP2-30437]
Immunocytochemistry/Immunofluorescence: MIDN Antibody [NBP2-30437] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.Immunohistochemistry: MIDN Antibody [NBP2-30437]
Immunohistochemistry: MIDN Antibody [NBP2-30437] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.Applications for MIDN Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MIDN
Alternate Names
DKFZp547M072, Midbrain nucleolar protein, midnolin
Gene Symbol
MIDN
UniProt
Additional MIDN Products
Product Documents for MIDN Antibody
Product Specific Notices for MIDN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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