Migfilin Antibody (5E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00054751-M10

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Migfilin Antibodies »

Product Specifications

Immunogen

FBLIM1 (NP_060026, 270 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTCARCIGDESFALGSQNEVYCLDDFYRKFAPVCSICENPIIPRDGKDAFKIECMGRNFHENCYRCEDCRILLSVEPTDQGCYPLNNHLFCKPCHVKRSAAGCC

Reactivity Notes

Human. Other species not tested.

Specificity

FBLIM1 - filamin binding LIM protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Migfilin Antibody (5E11)

Western Blot: Migfilin Antibody (5E11) [H00054751-M10]

Western Blot: Migfilin Antibody (5E11) [H00054751-M10]

Western Blot: Migfilin Antibody (5E11) [H00054751-M10] - Analysis of FBLIM1 expression in HepG2 (Cat # L019V1).
Western Blot: Migfilin Antibody (5E11) [H00054751-M10]

Western Blot: Migfilin Antibody (5E11) [H00054751-M10]

Western Blot: Migfilin Antibody (5E11) [H00054751-M10] - Analysis of FBLIM1 expression in transfected 293T cell line by FBLIM1 monoclonal antibody (M10), clone 5E11. Lane 1: FBLIM1 transfected lysatE (41 KDa). Lane 2: Non-transfected lysate.
ELISA: Migfilin Antibody (5E11) [H00054751-M10]

ELISA: Migfilin Antibody (5E11) [H00054751-M10]

ELISA: Migfilin Antibody (5E11) [H00054751-M10] - Detection limit for recombinant GST tagged FBLIM1 is approximately 0.03ng/ml as a capture antibody.

Applications for Migfilin Antibody (5E11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Migfilin

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CAL, DKFZp434G171, FBLP-1FBLP1CSX-associated LIM, filamin binding LIM protein 1, filamin-binding LIM protein 1, filamin-binding LIM protein-1, MIG2-interacting protein, MIGFILIN, mitogen-inducible 2 interacting protein, Mitogen-inducible 2-interacting protein

Entrez Gene IDs

54751 (Human)

Gene Symbol

FBLIM1

OMIM

607747 (Human)

UniProt

NP_060026 (Human)

Additional Migfilin Products

Product Documents for Migfilin Antibody (5E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Migfilin Antibody (5E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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