Migfilin Antibody (FBLIM1/4600)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13746

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FBLIM1/4600

Concentration

0.2 mg/ml

View all Migfilin Antibodies »

Product Summary for Migfilin Antibody (FBLIM1/4600)

Immunogen

Recombinant full-length human Migfilin protein (Uniprot: Q8WUP2)

Localization

Cytoplasm

Specificity

Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14149)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Migfilin Antibody (FBLIM1/4600)

Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Migfilin antibody (FBLIM1/4600) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Immunohistochemistry-Paraffin: Migfilin Antibody (FBLIM1/4600) [NBP3-13746] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Migfilin antibody (FBLIM1/4600) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Protein Array: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Protein Array: Migfilin Antibody (FBLIM1/4600) [NBP3-13746]

Protein Array: Migfilin Antibody (FBLIM1/4600) [NBP3-13746] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Migfilin antibody (FBLIM1/4600)

Applications for Migfilin Antibody (FBLIM1/4600)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Migfilin

FBLIM1 encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CAL, DKFZp434G171, FBLP-1FBLP1CSX-associated LIM, filamin binding LIM protein 1, filamin-binding LIM protein 1, filamin-binding LIM protein-1, MIG2-interacting protein, MIGFILIN, mitogen-inducible 2 interacting protein, Mitogen-inducible 2-interacting protein

Gene Symbol

FBLIM1

UniProt

Q8WUP2 (Human)

Additional Migfilin Products

Product Documents for Migfilin Antibody (FBLIM1/4600)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Migfilin Antibody (FBLIM1/4600)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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