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Migfilin Antibody (FBLIM1/8189R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20310

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # FBLIM1/8189R

Concentration

0.2 mg/ml

Product Summary for Migfilin Antibody (FBLIM1/8189R)

Immunogen

Recombinant full-length human Migfilin protein

Localization

Cytoplasm.

Specificity

This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human heart or kidney tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Migfilin Antibody (FBLIM1/8189R)

Migfilin Antibody (FBLIM1/8189R)

Migfilin Antibody (FBLIM1/8189R)

Formalin-fixed, paraffin-embedded human stomach stained with Migfilin antibody (FBLIM1/8189R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Migfilin Antibody (FBLIM1/8189R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Migfilin

FBLIM1 encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CAL, DKFZp434G171, FBLP-1FBLP1CSX-associated LIM, filamin binding LIM protein 1, filamin-binding LIM protein 1, filamin-binding LIM protein-1, MIG2-interacting protein, MIGFILIN, mitogen-inducible 2 interacting protein, Mitogen-inducible 2-interacting protein

Gene Symbol

FBLIM1

Additional Migfilin Products

Product Documents for Migfilin Antibody (FBLIM1/8189R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Migfilin Antibody (FBLIM1/8189R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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