Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Migfilin Antibodies »

Product Summary for Migfilin Antibody (OTI3G1) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-242 of human FBLIM1(NP_060026) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Migfilin Antibody (OTI3G1) - Azide and BSA Free

Western Blot: Migfilin Antibody (OTI3G1)Azide and BSA Free [NBP2-72691]

Western Blot: Migfilin Antibody (OTI3G1)Azide and BSA Free [NBP2-72691]

Western Blot: Migfilin Antibody (OTI3G1) - Azide and BSA Free [NBP2-72691] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Migfilin.
Western Blot: Migfilin Antibody (OTI3G1)Azide and BSA Free [NBP2-72691]

Western Blot: Migfilin Antibody (OTI3G1)Azide and BSA Free [NBP2-72691]

Western Blot: Migfilin Antibody (OTI3G1) - Azide and BSA Free [NBP2-72691] - Analysis of extracts (10ug) from 2 different cell lines.

Applications for Migfilin Antibody (OTI3G1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Migfilin

FBLIM1 encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CAL, DKFZp434G171, FBLP-1FBLP1CSX-associated LIM, filamin binding LIM protein 1, filamin-binding LIM protein 1, filamin-binding LIM protein-1, MIG2-interacting protein, MIGFILIN, mitogen-inducible 2 interacting protein, Mitogen-inducible 2-interacting protein

Gene Symbol

FBLIM1

Additional Migfilin Products

Product Documents for Migfilin Antibody (OTI3G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Migfilin Antibody (OTI3G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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