Migfilin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86665

Novus Biologicals, part of Bio-Techne
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NBP1-86665
NBP1-86665-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Migfilin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CEPCYQDTLERCGKCGEVVRDHIIRALGQAFHPSCFTCVTCARCIGDESFALGSQNEVYCLDDFYRKFAPVCSICENPIIPRDGKDAFKIECMGRNFHENCYRCEDCRILLSVEPTDQGCYPLNNHLFCKPCHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Migfilin Antibody

Western Blot: Migfilin Antibody [NBP1-86665]

Western Blot: Migfilin Antibody [NBP1-86665]

Western Blot: Migfilin Antibody [NBP1-86665] - Analysis in human cell lines A-431 and MCF-7 using Anti-FBLIM1 antibody. Corresponding FBLIM1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665]

Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665]

Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665]

Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665]

Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
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Applications for Migfilin Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Migfilin

FBLIM1 encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CAL, DKFZp434G171, FBLP-1FBLP1CSX-associated LIM, filamin binding LIM protein 1, filamin-binding LIM protein 1, filamin-binding LIM protein-1, MIG2-interacting protein, MIGFILIN, mitogen-inducible 2 interacting protein, Mitogen-inducible 2-interacting protein

Gene Symbol

FBLIM1

Additional Migfilin Products

Product Documents for Migfilin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Migfilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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