Mind Bomb 1/MIB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11304

Novus Biologicals, part of Bio-Techne
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NBP3-11304-0.025mg
NBP3-11304-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mind Bomb 1/MIB1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence C-DKDNTNVNADVQKLQ from the internal region of MIB1 (NP_065825.1)

Reactivity Notes

Predicted cross-reactivities: Rat, Mouse, Dog, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mind Bomb 1/MIB1 Antibody

Western Blot: Mind Bomb 1/MIB1 Antibody [NBP3-11304]

Western Blot: Mind Bomb 1/MIB1 Antibody [NBP3-11304]

Western Blot: Mind Bomb 1/MIB1 Antibody [NBP3-11304] - Analysis of HEK293 cell lysate probed with Goat anti Human MIB1 antibody: Lane A - overexpressing Myc tagged human MIB1. Lane B - mock transfected HEK293 cell lysate. Lane C - overexpressing Myc tagged human MIB1 probed with anti Myc tag antibody.
Immunohistochemistry-Paraffin: Mind Bomb 1/MIB1 Antibody [NBP3-11304]

Immunohistochemistry-Paraffin: Mind Bomb 1/MIB1 Antibody [NBP3-11304]

Immunohistochemistry-Paraffin: Mind Bomb 1/MIB1 Antibody [NBP3-11304] - FFPE human cerebral cortex stained with goat anti Human MIB1 following heat mediated antigen retrieval in citrate buffer pH 6.0.

Applications for Mind Bomb 1/MIB1 Antibody

Application
Recommended Usage

ELISA

1:16000

Western Blot

0.5 - 1.0 mg/mL
Application Notes
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

IHC-P: This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris buffered saline, 0.5% BSA

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mind Bomb 1/MIB1

MIB1 (Mind bomb 1; also DIP-1 and ZZANK2) is a 110-115 kDa E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non-redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand-expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand-expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006 amino acids (aa) in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc-finger motifs. There is one potential alternative start site at Met84. Over aa 365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.

Long Name

Mindbomb Homolog 1

Alternate Names

DIP1, MIB1, ZZANK2

Gene Symbol

MIB1

UniProt

Q86YT6 (Human)

Additional Mind Bomb 1/MIB1 Products

Product Documents for Mind Bomb 1/MIB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mind Bomb 1/MIB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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