Mindin/Spondin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30500

Novus Biologicals, part of Bio-Techne
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NBP2-30500
NBP2-30500-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Mindin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HPANSFYYPRLKALPPIARVTLLRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mindin/Spondin-2 Antibody

Western Blot: Mindin/Spondin-2 Antibody [NBP2-30500]

Western Blot: Mindin/Spondin-2 Antibody [NBP2-30500]

Western Blot: Mindin/Spondin-2 Antibody [NBP2-30500] - Analysis in control (vector only transfected HEK293T lysate) and SPON2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500]

Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500]

Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500] - Staining of human placenta shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500]

Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500]

Immunohistochemistry-Paraffin: Mindin/Spondin-2 Antibody [NBP2-30500] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
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Applications for Mindin/Spondin-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mindin

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens . Binds bacterial lipopolysaccharide (LPS)

Alternate Names

DIL1, RG-1, SPON2, Spondin 2

Gene Symbol

SPON2

UniProt

Q9BUD6

Additional Mindin Products

Product Documents for Mindin/Spondin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mindin/Spondin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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