MITF Antibody (S06-4B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19691

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19691-100ul
NBP3-19691-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-4B5

Concentration

0.3 mg/ml

View all MITF Antibodies »

Product Summary for MITF Antibody (S06-4B5)

Immunogen

Recombinant protein of human MITF (Uniprot # O75030)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MITF Antibody (S06-4B5)

Western Blot: MITF Antibody (S06-4B5) [NBP3-19691]

Western Blot: MITF Antibody (S06-4B5) [NBP3-19691]

Western Blot: MITF Antibody (S06-4B5) [NBP3-19691] - Western blot detection of MITF in Hela, CHO-K1, Jurkat cell lysates using NBP3-19691 (1:500 diluted). Predicted band size: 59kDa. Observed band size: 52kDa.

Applications for MITF Antibody (S06-4B5)

Application
Recommended Usage

Western Blot

1/1000-1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

Additional MITF Products

Product Documents for MITF Antibody (S06-4B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MITF Antibody (S06-4B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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