MITF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88618

Novus Biologicals, part of Bio-Techne
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NBP1-88618
NBP1-88618-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MITF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MITF Antibody

Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618]

Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618]

Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618] - Analysis in human skin and pancreas tissues using NBP1-88618 antibody. Corresponding MITF RNA-seq data are presented for the same tissues.
Western Blot: MITF Antibody [NBP1-88618]

Western Blot: MITF Antibody [NBP1-88618]

Western Blot: MITF Antibody [NBP1-88618] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-MITF antibody. Corresponding MITF RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618]

Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618]

Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
View 3 more images

Applications for MITF Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
ChIP reported in scientific literature (PMID: 24443471). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

Additional MITF Products

Product Documents for MITF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MITF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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