Mitochondria Antibody (113-1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34517
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 113-1
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Semi-Purified mitochondrial preparation
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Mitochondria in cytoplasm
Specificity
This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.
Marker
Marker for Human Cells, Granular RCC s & Salivary Tumor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32980).
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Mitochondria Antibody (113-1) - Azide and BSA Free
Western Blot: Mitochondria Antibody (113-1)Azide and BSA Free [NBP2-34517]
Western Blot: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Western Blot analysis of HeLa cell lysate using Mitochondria Antibody (113-1).Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517]
Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Confocal immunofluorescence image of HeLa cells using labeled is Green (CF488) and Red Dot is used to label the nuclei Red.Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517]
Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Biotin conjugated Mitochondria expression in human prostate cancer tissue (left) and mouse stomach tissue (right). Image from verified customer review.Applications for Mitochondria Antibody (113-1) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Frozen
0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (113-1) - Azide and BSA Free
Product Specific Notices for Mitochondria Antibody (113-1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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