Mitochondria Antibody (AE-1) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48282
![Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-AE-1-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48282-img0002.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AE-1
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Mitochondria Antibody (AE-1) - IHC-Prediluted
Immunogen
Semi-Purified mitochondrial preparation
Reactivity Notes
Does not react with Mouse and Rat.
Localization
Mitochondria in cytoplasm
Specificity
monoclonal antibody MTC02 recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody MTC02 recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. monoclonal antibody MTC02 is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Marker
Marker for Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Mitochondria Antibody (AE-1) - IHC-Prediluted
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (AE-1).![Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-AE-1-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48282-img0001.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]")
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282]
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Tonsil stained with Mitochondria Mouse Monoclonal Antibody (AE-1).Applications for Mitochondria Antibody (AE-1) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (AE-1) - IHC-Prediluted
Product Specific Notices for Mitochondria Antibody (AE-1) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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