Mitochondria Antibody (MTC754)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32874

Novus Biologicals, part of Bio-Techne
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NBP2-32874-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MTC754

Concentration

0.2 mg/ml

View all Mitochondria Antibodies »

Product Summary for Mitochondria Antibody (MTC754)

Immunogen

Mitochondrial fraction of HeLa cells

Reactivity Notes

Shows broad species reactivity including Insects or Bacteria.

Localization

Mitochondria in cytoplasm

Specificity

This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Marker

Mitochondrial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34688)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Mitochondria Antibody (MTC754)

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874]

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874]

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (MTC754).
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874]

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874]

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC754) [NBP2-32874] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC754).

Applications for Mitochondria Antibody (MTC754)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Mitochondria

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Additional Mitochondria Products

Product Documents for Mitochondria Antibody (MTC754)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mitochondria Antibody (MTC754)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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