Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72708
![Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]](https://resources.bio-techne.com/images/products/Mitocondrial-Translational-Initiation-Factor-3-Antibody-OTI4B6-Azide-and-BSA-Free-Western-Blot-NBP2-72708-img0002.jpg "Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Canine, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI4B6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 56-278 of human MTIF3 (NP_690876) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]
Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6) - Azide and BSA Free [NBP2-72708] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTIF3.![Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]](https://resources.bio-techne.com/images/products/Mitocondrial-Translational-Initiation-Factor-3-Antibody-OTI4B6-Azide-and-BSA-Free-Western-Blot-NBP2-72708-img0001.jpg "Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]")
Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6)Azide and BSA Free [NBP2-72708]
Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody (OTI4B6) - Azide and BSA Free [NBP2-72708] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Applications
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Mitocondrial Translational Initiation Factor 3
Alternate Names
FLJ33676, IF3(mt), IF-3mt, mitochondrial, mitochondrial translational initiation factor 3
Gene Symbol
MTIF3
Additional Mitocondrial Translational Initiation Factor 3 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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