Mitofusin 2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66383
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human MFN2 protein (amino acids 364-599). [O95140]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mitofusin 2 Antibody - BSA Free
Western Blot: Mitofusin 2 AntibodyBSA Free [NBP2-66383]
Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody - BSA Free [NBP2-66383]
Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody [NBP2-66383] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Mitofusin 2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: Mitofusin 2 Antibody - BSA Free [NBP2-66383]
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-66383] - Analysis of FFPE tissue section of mouse heart using Mitofusin 2/MFN2 antibody at 5 ug/mL concentration (1:200 dilution). The primary antibody binding to MFN2 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This MFN2 antibody generated a diffused to punctate cytoplasmic staining of mitochondria in the cardiac muscles (brown color). The nuclei of the muscle cells and the macrophages are stained blue in this image (40X image of a transverse section of heart tissue).Applications for Mitofusin 2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 - 10 ug/mL
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
0.5 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Mitofusin 2
Alternate Names
CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2
Gene Symbol
MFN2
Additional Mitofusin 2 Products
Product Documents for Mitofusin 2 Antibody - BSA Free
Product Specific Notices for Mitofusin 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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