MKL2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46209
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Monkey
Cited:
Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1038 and 1088 of human Megakaryoblastic Leukemia 2 using the numbering given in entry NP_054767.3 (GeneID 57496).
Reactivity Notes
Monkey reactivity reported in scientific literature (PMID:31801086).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MKL2 Antibody
![Western Blot: MKL2 Antibody [NBP1-46209]](https://resources.bio-techne.com/images/products/MKL2-Antibody-Western-Blot-NBP1-46209-img0002.jpg "Western Blot: MKL2 Antibody [NBP1-46209]")
Western Blot: MKL2 Antibody [NBP1-46209]
Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).![Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209]](https://resources.bio-techne.com/images/products/MKL2-Antibody-Immunohistochemistry-NBP1-46209-img0004.jpg "Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209]")
Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209]
Immunohistochemistry-Paraffin: MKL2 Antibody [NBP1-46209] - Section of human prostate carcinoma and linitis plastica stomach cancer . Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DABApplications for MKL2 Antibody
Application
Recommended Usage
Immunoblotting
Reactivity reported in (PMID: 28592291).
Immunocytochemistry/ Immunofluorescence
Reactivity reported in (PMID: 28592291).
Immunohistochemistry
1:200 -1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MKL2
Alternate Names
DKFZp686J1745, FLJ31823, FLJ45623, KIAA1243, megakaryoblastic leukemia 2, MKL/myocardin-like 2, MKL/myocardin-like protein 2, MRTFB, MRTF-B, myocardin-related transcription factor B, NPD001
Gene Symbol
MRTFB
UniProt
Additional MKL2 Products
Product Documents for MKL2 Antibody
Product Specific Notices for MKL2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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