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MKLP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17299

Novus Biologicals, part of Bio-Techne
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NBP2-17299

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MKLP1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKLP1 Antibody

Immunocytochemistry/ Immunofluorescence: MKLP1 Antibody [NBP2-17299]

Immunocytochemistry/ Immunofluorescence: MKLP1 Antibody [NBP2-17299]

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Immunocytochemistry/ Immunofluorescence: MKLP1 Antibody [NBP2-17299]

Immunocytochemistry/ Immunofluorescence: MKLP1 Antibody [NBP2-17299]

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
MKLP1 Antibody

Western Blot: MKLP1 Antibody [NBP2-17299] -

Western Blot: MKLP1 Antibody [NBP2-17299] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MKLP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKLP1

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms. [provided by RefSeq]

Alternate Names

kinesin family member 23, kinesin-like 5 (mitotic kinesin-like protein 1), Kinesin-like protein 5, kinesin-like protein KIF23, KNSL5CHO1, mitotic kinesin-like 1, MKLP-1, MKLP1Mitotic kinesin-like protein 1

Gene Symbol

KIF23

UniProt

Additional MKLP1 Products

Product Documents for MKLP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKLP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.