MKP-3/DUSP6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26174

Novus Biologicals, part of Bio-Techne
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NBP2-26174
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MKP-3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PSNQNVYQVDSLQST corresponding to C-Terminus according to NP_073143.2.

Specificity

This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MKP-3/DUSP6 Antibody

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174]

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174]

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174] - Human (A), (Mouse) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174]

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174]

Western Blot: MKP-3/DUSP6 Antibody [NBP2-26174] - Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MKP-3/DUSP6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in mouse and rat heart lysates and 40 kDa band plus 26 kDa band bands observed in human and porcine heart lysates (calculated MW of 42.3 kDa band according to NP_001937.2 and 26.5 kDa band according to NP_073143.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKP-3

DUSP6 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

MAP Kinase Phosphatase 3

Alternate Names

DUSP6, MKP3, PYST1

Entrez Gene IDs

1848 (Human); 67603 (Mouse); 116663 (Rat)

Gene Symbol

DUSP6

Additional MKP-3 Products

Product Documents for MKP-3/DUSP6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MKP-3/DUSP6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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