MKRN1 Antibody (OTI2C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45816

Novus Biologicals, part of Bio-Techne
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NBP2-45816
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-72718AF350
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Alexa Fluor 405
NBP2-72718AF405
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Alexa Fluor 488
NBP2-72718AF488
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Alexa Fluor 532
NBP2-72718AF532
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Alexa Fluor 594
NBP2-72718AF594
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Alexa Fluor 647
NBP2-72718AF647
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Alexa Fluor 700
NBP2-72718AF700
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Alexa Fluor 750
NBP2-72718AF750
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Allophycocyanin
NBP2-72718APC
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Allophycocyanin/Cy7
NBP2-72718APCCY7
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Biotin
NBP2-72718B
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CoraFluor 1
NBP2-72718CL1
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DyLight 350
NBP2-72718UV
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DyLight 405
NBP2-72718V
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DyLight 488
NBP2-72718G
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DyLight 550
NBP2-72718R
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DyLight 594
NBP2-72718DL594
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DyLight 650
NBP2-72718C
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DyLight 680
NBP2-72718FR
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DyLight 755
NBP2-72718IR
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FITC
NBP2-72718F
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HRP
NBP2-72718H
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Janelia Fluor 525
NBP2-72718JF525
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Janelia Fluor 549
NBP2-72718JF549
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Janelia Fluor 585
NBP2-72718JF585
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Janelia Fluor 635
NBP2-72718JF635
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Janelia Fluor 646
NBP2-72718JF646
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Janelia Fluor 669
NBP2-72718JF669
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mFluor Violet 450 SE
NBP2-72718MFV450
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mFluor Violet 500 SE
NBP2-72718MFV500
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mFluor Violet 610 SE
NBP2-72718MFV610
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PE
NBP2-72718PE
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PE/Atto594
NBP2-72718PEATT594
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PE/Cy5.5
NBP2-72718PECY55
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PE/Cy7
NBP2-72718PECY7
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PerCP
NBP2-72718PCP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C8

Concentration

1 mg/ml

View all MKRN1 Antibodies »

Product Specifications

Immunogen

Full length human recomnbinant protein of human MKRN1(NP_038474) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKRN1 Antibody (OTI2C8)

Western Blot: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Western Blot: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Western Blot: MKRN1 Antibody (OTI2C8) [NBP2-45816] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1.
Immunohistochemistry: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Immunohistochemistry: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Immunohistochemistry: MKRN1 Antibody (OTI2C8) [NBP2-45816] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Flow Cytometry: MKRN1 Antibody (OTI2C8) [NBP2-45816]

Flow Cytometry: MKRN1 Antibody (OTI2C8) [NBP2-45816] - Analysis of Jurkat cells, using anti-MKRN1 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).
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Applications for MKRN1 Antibody (OTI2C8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKRN1

MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.

Alternate Names

E3 ubiquitin-protein ligase makorin-1, EC 6.3.2.-, FLJ21334, makorin ring finger protein 1, RNF61RING finger protein 61

Gene Symbol

MKRN1

Additional MKRN1 Products

Product Documents for MKRN1 Antibody (OTI2C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKRN1 Antibody (OTI2C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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