MLC1SA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74247

Novus Biologicals, part of Bio-Techne
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NBP1-74247
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all MLC1SA Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a region of MLC1SA. Peptide sequence: PKKDAPVKKPAGPSISKPAAKSTAGTPLAKTKAEPAAPQTPVKTQEPPVD.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MLC1SA Antibody

Western Blot: MLC1SA Antibody [NBP1-74247]

Western Blot: MLC1SA Antibody [NBP1-74247]

Western Blot: MLC1SA Antibody [NBP1-74247] - Titration: 1.0 ug/ml. Positive Control: RPMI-8226.
Western Blot: MLC1SA Antibody [NBP1-74247]

Western Blot: MLC1SA Antibody [NBP1-74247]

Western Blot: LOC100359980 Antibody [NBP1-74247] - Rat Liver Lysate 1ug/ml Gel Concentration 12%

Applications for MLC1SA Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLC1SA

MYL6B is a heavy chain regulator found in smooth muscle and non-muscle Myosin complexes. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain by Myosin light chain kinase. MYL6B does not bind calcium during contraction. It is primarily found in a hexamer consisting of four light chains and two heavy chains. It most commonly interacts with Myosin Va, an Actin based motor that can move in large steps. MYL6B is expressed in most tissues with neurons and smooth muscle tissue having the highest expression. Recombinant human MYL6B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

MLC1sa, MLC1SAmyosin light chain 1, slow-twitch muscle A isoform, myosin alkali light chain 1 slow a, myosin light chain 1 slow a, Myosin light chain 1 slow-twitch muscle A isoform, myosin light chain 6B, myosin, light chain 6B, alkali, smooth muscle and non-muscle, myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle, smooth muscle and non-muscle myosin alkali light chain 6B, Smooth muscle and nonmuscle myosin light chain alkali 6B

Gene Symbol

MYL6B

UniProt

D3ZHA7 (Rat)

Additional MLC1SA Products

Product Documents for MLC1SA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MLC1SA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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