MLH3 Antibody (CL14084)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24596

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14084

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MLH3 Antibodies »

Product Summary for MLH3 Antibody (CL14084)

Immunogen

This antibody was designed to target the recombinant protein MLH3, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MLH3 Antibody (CL14084)

Immunohistochemistry-Paraffin: MLH3 Antibody (CL14084) [NBP3-24596]

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MLH3 Antibody (CL14084) [NBP3-24596]

Analysis in human endometrium and skeletal muscle tissues using NBP3-24596 antibody. Corresponding MLH3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MLH3 Antibody (CL14084) [NBP3-24596]

Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
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Applications for MLH3 Antibody (CL14084)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLH3

MLH3 is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Alternate Names

DNA mismatch repair protein Mlh3, HNPCC7, MGC138372, mutL (E. coli) homolog 3, mutL homolog 3 (E. coli), MutL protein homolog 3

Gene Symbol

MLH3

Additional MLH3 Products

Product Documents for MLH3 Antibody (CL14084)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MLH3 Antibody (CL14084)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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